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Relative mRNA levels of VEGF, HIF-1α, and MMP2 were quantified in placental villous tissue from both RSA patients and the control group. (A) mRNA expressions of VEGF, HIF-1α, and MMP2 were determined in the placental villous tissue using RT qPCR. GAPDH was used as an internal control. (B) mRNA levels were assessed by <t>RT-PCR.</t> VEGF, vascular endothelial growth factor; RSA, recurrent spontaneous abortion; HIF-1α, hypoxia-inducible factor-1α; MMP2, matrix metalloproteinase-2; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; mRNA, messenger RNA; RT qPCR, real-time quantitative <t>polymerase</t> chain reaction; RT-PCR, real-time polymerase chain reaction. * P <0.05 vs. control group.
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Relative mRNA levels of VEGF, HIF-1α, and MMP2 were quantified in placental villous tissue from both RSA patients and the control group. (A) mRNA expressions of VEGF, HIF-1α, and MMP2 were determined in the placental villous tissue using RT qPCR. GAPDH was used as an internal control. (B) mRNA levels were assessed by RT-PCR. VEGF, vascular endothelial growth factor; RSA, recurrent spontaneous abortion; HIF-1α, hypoxia-inducible factor-1α; MMP2, matrix metalloproteinase-2; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; mRNA, messenger RNA; RT qPCR, real-time quantitative polymerase chain reaction; RT-PCR, real-time polymerase chain reaction. * P <0.05 vs. control group.

Journal: Obstetrics & Gynecology Science

Article Title: Correlation of VEGF, HIF-1α, and MMP2 expression in placental villi among patients with recurrent spontaneous abortion

doi: 10.5468/ogs.24176

Figure Lengend Snippet: Relative mRNA levels of VEGF, HIF-1α, and MMP2 were quantified in placental villous tissue from both RSA patients and the control group. (A) mRNA expressions of VEGF, HIF-1α, and MMP2 were determined in the placental villous tissue using RT qPCR. GAPDH was used as an internal control. (B) mRNA levels were assessed by RT-PCR. VEGF, vascular endothelial growth factor; RSA, recurrent spontaneous abortion; HIF-1α, hypoxia-inducible factor-1α; MMP2, matrix metalloproteinase-2; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; mRNA, messenger RNA; RT qPCR, real-time quantitative polymerase chain reaction; RT-PCR, real-time polymerase chain reaction. * P <0.05 vs. control group.

Article Snippet: The polymerase chain reaction (PCR) reverse transcription into cDNA (PrimeScript TM RT Reagent kit; Takara Bio Inc., Shiga, Japan) and PCR amplification kits (SYBR ® Premix Ex TaqTM II; Takara Bio Inc.) were purchased from TaKaRa (Takara Bio Inc.).

Techniques: Control, Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction